Mapping Local Conformational Landscapes of Proteins in Solution
نویسندگان
چکیده
منابع مشابه
Understanding how small helical proteins fold: conformational dynamics of Im proteins relevant to their folding landscapes.
Understanding the mechanism of folding of small proteins requires characterization of their starting unfolded states and any partially unfolded states populated during folding. Here, we review what is known from NMR about these states of Im7, a 4-helix bundle protein that folds via an on-pathway intermediate, and show that there is an alignment of non-native structure in urea-unfolded Im7 with ...
متن کاملLocal Conformational Changes in the DNA Interfaces of Proteins
When a protein binds to DNA, a conformational change is often induced so that the protein will fit into the DNA structure. Therefore, quantitative analyses were conducted to understand the conformational changes in proteins. The results showed that conformational changes in DNA interfaces are more frequent than in non-interfaces, and DNA interfaces have more conformational variations in the DNA...
متن کاملSolution conformational analysis of the alpha-zein proteins of maize.
Small angle x-ray scattering and viscometric analyses of the alpha-zeins of maize in solution indicated that the molecules were asymmetric. Structure predictions of consensus sequences for the two classes of alpha-zeins, Z19 and Z22, were in good agreement with the alpha-helical contents determined by circular dichroism. Dimensions determined by small angle x-ray scattering and viscometry indic...
متن کاملNMR mapping of protein conformational landscapes using coordinated behavior of chemical shifts upon ligand binding.
Proteins exist as an ensemble of conformers that are distributed on free energy landscapes resembling folding funnels. While the most stable conformers populate low energy basins, protein function is often carried out through low-populated conformational states that occupy high energy basins. Ligand binding shifts the populations of these states, changing the distribution of these conformers. U...
متن کاملMapping Twitter Conversation Landscapes
While the most ambitious polls are based on standardized interviews with a few thousand people, millions are tweeting freely and publicly in their own voices about issues they care about. This data offers a vibrant 24/7 snapshot of people’s response to various events and topics. The sheer scale of the data on Twitter allows us to measure in aggregate how the various issues are rising and fallin...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Structure
سال: 2019
ISSN: 0969-2126
DOI: 10.1016/j.str.2019.03.005